Find methods for your needs

Refine by Feature

More...
Name
More...
Class
More...
Manufacturer
Model
Stationary Phase
Dimensions
Length
Diameter/Tubing Size ID
Tubing Size OD
Particle Size/Film Thickness

Displaying 1-12 of 12 results for: charge variant

Items per page:
Sort by

Simple charge variant profile comparison of an innovator monoclonal antibody and a biosimilar candidate

Instrument Type: UHPLC

To demonstrate the effectiveness of a simple pH gradient/ion-exchange chromatography workflow approach to the characterization of charge variant profiles of an innovator molecule (cetuximab) and a candidate biosimilar. To show the assay is simple, reproducible, easily optimized and resolves variants effectively.

Evaluation and application of salt- and pH-based ion-exchange chromatography gradients for analysis of therapeutic monoclonal antibodies

Instrument Type: UHPLC

To demonstrate the CX-1 pH buffer system is capable of charge variant determination for the majority of therapeutic monoclonal antibody species. To show the ease of optimization and improved reproducibility of this buffer system when compared to salt-based gradient systems used for charge variant analysis.

High resolution charge variants analysis of recombinant human IgG1K NIST mAb

Instrument Type: HPLC-CAD

The Thermo Scientific Vanquish Flex UHPLC system with UV detection is applied for the charge variants analysis of NISTmAb Humanized IgG1K monoclonal antibody using strong cation exchange-based chromatographic separation on a MAbPac SCX-10 column. The use of Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of reference material monoclonal antibody charge variants using a 30 minute gradient.

High resolution charge variant analysis of Cetuximab by ion exchange chromatography using pH gradient buffers

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific MAbPac SCX-10 column is applied to the charge variant analysis of Cetuximab. Ion exchange separation was performed using a pH gradient on a Thermo Scientific Vanquish Flex UHPLC system with UV detection at 280 nm. Use of Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of monoclonal antibody charge variants over a 30 minute gradient.

High resolution charge variant analysis of Infliximab by ion exchange chromatography using pH gradient buffers

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific MAbPac SCX-10 column is applied for charge variant analysis of Infliximab. Ion exchange separation was performed on a Vanquish Flex UHPLC system using a pH gradient and UV detection at 280 nm. Use of Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of monoclonal antibody charge variants over a 30 minute gradient.

High resolution charge variant analysis of Bevacizumab by ion exchange chromatography using pH gradient buffers

Instrument Type: HPLC (Biocompatible)

Ion exchange separation is applied for charge variant analysis of Bevacizumab using a pH gradient on a Thermo Scientific MAbPac SCX-10 column. The Thermo Scientific Vanquish Flex UHPLC system with UV detection at 280 nm was used with Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of monoclonal antibody charge variants over a 30 minute gradient.

Separation of Acidic and Basic charge variants of Denosumab by Ion Exchange chromatography using pH Gradient Buffers

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the Ion Exchange analysis of Denosumab. The separation was performed on a Thermo Scientific MAbPac SCX-10 and pH gradient buffers with a UV detection at 280 nm. With a run time of 50 minutes the method allows easy separation of charge variants of Denosumab.

Separation of Acidic and Basic charge variants of Denosumab by Ion Exchange chromatography using Salt Gradient Method

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the Ion Exchange analysis of Denosumab. The separation was performed on a Thermo Scientific MabPac SCX-10 column with a UV detection at 220 nm. With a run time of 65 minutes the method allows easy separation of charge variants of Denosumab.

Faster separation of acidic and basic charge variants of Rituximab by Ion Exchange Chromatography using a shorter salt gradient method.

Instrument Type: UHPLC

The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the Ion Exchange analysis of Rituximab. The separation was performed on a 3 micron Thermo Scientific MabPac SCX-10 column with a UV detection at 280 nm. With a run time of 16 minutes, the method allows easy separation of acidic and basic charge variants of Rituximab

pH gradient with Vanquish Flex UHPLC system and MAbPAc SCX-10

Instrument Type: HPLC (Biocompatible)

Charge variants analysis by ion exchange chromatography is one of the major analyses in biotherapeutics characterization. Here we show charge variant analysis of a therapeutic antibody with The Thermo Scientific Vanquish Flex UHPLC in combination with the Thermo Scientific MAbPac SCX-10, 5 µm. The ion exchange separation was performed in pH gradient mode by usingthe Thermo Scientific CX-1 pH-Gradient buffers.

Fast pH gradient separation of cetuximab charge variants

Instrument Type: Unspecified

The Thermo Scientific Ultimate 3000 BioRS Dual system is used in combination with the Thermo Scientific MabPac SCX-10 RS 2.1x50mm to run fast pH-gradient ion exchange of cetuximab. The pH gradient is generated by using the CX-1 pH-gradient buffers.A fast generic screening method is run with a 10 minutes gradient from pH 5,6 to pH 10,2. The generic screening method isoptimized to achieve high throughput, high resolution separation of the charge variants.

Development of ultra-fast pH gradient ion exchange chromatography for the separation of monoclonal antibody charge variants

Instrument Type: UHPLC

The Thermo Scientific Vanquish UHPLC system is used in combination with the Thermo Scientific MabPac SCX-10 RS 2.1x50mm to run fast pH-gradient ion exchange of four monoclonal antibodies. The pH gradient is generated by using the CX-1 pH-gradient buffers. A fast generic screening method is run with a 10 minutes gradient from pH 5,6 to pH 10,2. The generic screening method was afterward optimized for each antibody to achieve high throughput, high resolution separation of the charge variants.