High resolution charge variant analysis of Bevacizumab by ion exchange chromatography using pH gradient buffers
DescriptionIon exchange separation is applied for charge variant analysis of Bevacizumab using a pH gradient on a Thermo Scientific MAbPac SCX-10 column. The Thermo Scientific Vanquish Flex UHPLC system with UV detection at 280 nm was used with Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of monoclonal antibody charge variants over a 30 minute gradient.
|Keywords:||Biotherapeutics, Monoclonal antibody, Bevacizumab, MabPac SCX-10, pH gradient buffer, charge variant analysis, ion exchange chromatography, NIBRT, Bioprocessing Research|
|Author:||Amy Farrell, Craig Jakes, Ken Cook and Jonathan Bones|
|Affiliation:||NIBRT, Dublin, Ireland|
For Research Use Only. Not for use in diagnostic procedures.