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High resolution charge variant analysis of Bevacizumab by ion exchange chromatography using pH gradient buffers Be the first to rate this application


Ion exchange separation is applied for charge variant analysis of Bevacizumab using a pH gradient on a Thermo Scientific MAbPac SCX-10 column. The Thermo Scientific Vanquish Flex UHPLC system with UV detection at 280 nm was used with Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of monoclonal antibody charge variants over a 30 minute gradient.
Market: BioPharma
Keywords: Biotherapeutics, Monoclonal antibody, Bevacizumab, MabPac SCX-10, pH gradient buffer, charge variant analysis, ion exchange chromatography, NIBRT, Bioprocessing Research
Matrix: Drug product
Author: Amy Farrell, Craig Jakes, Ken Cook and Jonathan Bones
Affiliation: NIBRT, Dublin, Ireland
Uploaded on 12/21/2016.

For Research Use Only. Not for use in diagnostic procedures.