LC Method Transfer Calculator – an online tool to transfer methods from HPLC to UHPLC conditions

Use at your own risk. Prepopulated data are estimates only. Thermo Fisher Scientific takes no responsibility for the accuracy of data, calculations or results.

Current Column

Length (mm):

Diameter (mm):

Particle Size (µm):

Planned Column

Length (mm):

Diameter (mm):

Particle Size (µm):

Peak Details (Critical Pair)

Actual R_s (Resolution Factor):

Peak Details (Critical Pair)

Predicted R_s Change Factor:

Predicted R_s:

Current Method Conditions

* Use this factor to increase the flow rate of the fast LC method. Note: if factor other than 1 is used, the resolution calculation is disabled.

Flow (mL/min):

Injection Volume (µL):

Max Observed Pressure:

Pressure Units:

Number of samples:

Data Collection Rate (Hz):

Consider Gradient Delay Volume (GDV)

Gradient Delay Volume (µL):

Recommended Method Conditions

Boost Factor:

* Use this factor to increase the flow rate of the fast LC method. Note: if factor other than 1 is used, the resolution calculation is disabled.

Boost Factor:

* Use this factor to increase the flow rate of the fast LC method. Note: if factor other than 1 is used, the resolution calculation is disabled.

Adjust Flow

Flow (mL/min):

Injection Volume (µL):

Estimated Max Observed Pressure:

Number of samples:

Data Collection Rate (Hz):

Gradient Delay Volume (µL):

Optimal GDV (µL):

Current Gradient Table

Step	Time (min)	%A	%B	%C	%D
1
	Cannot have more than 100% mix.
2
	Cannot have more than 100% mix.
3
	Cannot have more than 100% mix.
4
	Cannot have more than 100% mix.
5
	Cannot have more than 100% mix.
6
	Cannot have more than 100% mix.
7
	Cannot have more than 100% mix.
8
	Cannot have more than 100% mix.
9
	Cannot have more than 100% mix.
10
	Cannot have more than 100% mix.
End Time:
Recommended Reconditioning Time:

Planned Gradient Table

Step	Time (min)	%A	%B	%C	%D
1
	Cannot have more than 100% mix.
2
	Cannot have more than 100% mix.
3
	Cannot have more than 100% mix.
4
	Cannot have more than 100% mix.
5
	Cannot have more than 100% mix.
6
	Cannot have more than 100% mix.
7
	Cannot have more than 100% mix.
8
	Cannot have more than 100% mix.
9
	Cannot have more than 100% mix.
10
	Cannot have more than 100% mix.
End Time:
Recommended Reconditioning Time:

Eluent usage and time savings

	Current Total	Planned Total	Savings	Throughput
Eluent Usage
Time

Sample usage savings

Current Usage	Planned Usage	Savings