LC Method Transfer Calculator

LC Method Transfer Calculator – an online tool to transfer methods from HPLC to UHPLC conditions

LC Method Transfer Calculator – an online tool to transfer methods from HPLC to UHPLC conditions

Use at your own risk. Prepopulated data are estimates only. Thermo Fisher Scientific takes no responsibility for the accuracy of data, calculations or results.

Current Column
Planned Column
Peak Details (Critical Pair)
Peak Details (Critical Pair)
Current Method Conditions
* Use this factor to increase the flow rate of the fast LC method. Note: if factor other than 1 is used, the resolution calculation is disabled.
Recommended Method Conditions
* Use this factor to increase the flow rate of the fast LC method. Note: if factor other than 1 is used, the resolution calculation is disabled.
* Use this factor to increase the flow rate of the fast LC method. Note: if factor other than 1 is used, the resolution calculation is disabled.
Current Gradient Table
Step Time (min) %A %B %C %D
1
Cannot have more than 100% mix.
2
Cannot have more than 100% mix.
3
Cannot have more than 100% mix.
4
Cannot have more than 100% mix.
5
Cannot have more than 100% mix.
6
Cannot have more than 100% mix.
7
Cannot have more than 100% mix.
8
Cannot have more than 100% mix.
9
Cannot have more than 100% mix.
10
Cannot have more than 100% mix.
End Time:
Recommended Reconditioning Time:
Planned Gradient Table
Step Time (min) %A %B %C %D
1
Cannot have more than 100% mix.
2
Cannot have more than 100% mix.
3
Cannot have more than 100% mix.
4
Cannot have more than 100% mix.
5
Cannot have more than 100% mix.
6
Cannot have more than 100% mix.
7
Cannot have more than 100% mix.
8
Cannot have more than 100% mix.
9
Cannot have more than 100% mix.
10
Cannot have more than 100% mix.
End Time:
Recommended Reconditioning Time:
Eluent usage and time savings
Current Total Planned Total Savings Throughput
Eluent Usage
Time
Sample usage savings
Current Usage Planned Usage Savings