Find methods for your needs
Refine by Feature
Displaying 1-5 of 16 results for Tag: Biotherapeutics
TN72264: HPAE-PAD N-linked Oligosaccharide Profiling of IgGInstrument Type: IC
This technical note reports an HPAE-PAD method to profile IgG N-linked oligosaccharides and N-linked high-mannose type oligosaccharides that are atypical for human polyclonal IgG but sometimes found on mAbs. This HPAE-PAD method is orthogonal to other oligosaccharide methods applied to IgG. More information on the development and application of the separation described in the technical note can be found in Rohrer, et al. as referenced in the document.
High resolution charge variants analysis of recombinant human IgG1K NIST mAbInstrument Type: HPLC-CAD
The Thermo Scientific Vanquish Flex UHPLC system with UV detection is applied for the charge variants analysis of NISTmAb Humanized IgG1K monoclonal antibody using strong cation exchange-based chromatographic separation on a MAbPac SCX-10 column. The use of Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of reference material monoclonal antibody charge variants using a 30 minute gradient.
Ultrafast hydrophilic interaction UHPLC analysis of 2AA labelled N-glycans from monoclonal antibodies (mAbs)Instrument Type: UHPLC
The Thermo Scientific Vanquish UHPLC system with Fluorescence detection is applied for the rapid glyco-profiling of a commercial chimeric IgG1 mAb.The separation is performed on a Thermo Scientific Accucore-150-Amide HILIC column in under 2.5 minutes. The developed methodology offers an excellent analytical platform for ultrafast glycan profile comparison of different batches of these mAbs.
Tandem UHPLC operation for high-throughput LC-MS peptide mapping analysesInstrument Type: LCMS
To demonstrate the use of a multi-pump UHPLC system and enable tandem analysis with two columns in parallel, addressing productivity and throughput improvement of existing LC-MS methods.
High resolution charge variant analysis of Cetuximab by ion exchange chromatography using pH gradient buffersInstrument Type: HPLC (Biocompatible)
The Thermo Scientific MAbPac SCX-10 column is applied to the charge variant analysis of Cetuximab. Ion exchange separation was performed using a pH gradient on a Thermo Scientific Vanquish Flex UHPLC system with UV detection at 280 nm. Use of Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of monoclonal antibody charge variants over a 30 minute gradient.