Rate
Ask the Expert
Suggest an Application
Add to Favorites
Create
Parts List
Parts List
Tell a
Friend
Friend
Print
PDF
Find methods for your needs
High resolution charge variants analysis of recombinant human IgG1K NIST mAb
Description
The Thermo Scientific Vanquish Flex UHPLC system with UV detection is applied for the charge variants analysis of NISTmAb Humanized IgG1K monoclonal antibody using strong cation exchange-based chromatographic separation on a MAbPac SCX-10 column. The use of Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of reference material monoclonal antibody charge variants using a 30 minute gradient.Market: | BioPharma |
Keywords: | Biotherapeutics, MAbPac SCX-10 Column, Vanquish Flex UHPLC System, Biosimilars, pH gradient buffer, charge variant analysis, NISTmAb, reference material 8671, Humanized IgG1K, NIBRT, Bioprocessing Research |
Matrix: | NIST standard |
Author: | Silvia Millán, Amy Farrell and Jonathan Bones |
Affiliation: | NIBRT, Dublin, Ireland |
Status: | Proof of Concept |
For Research Use Only. Not for use in diagnostic procedures.