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High resolution charge variants analysis of recombinant human IgG1K NIST mAb Be the first to rate this application

Description

The Thermo Scientific Vanquish Flex UHPLC system with UV detection is applied for the charge variants analysis of NISTmAb Humanized IgG1K monoclonal antibody using strong cation exchange-based chromatographic separation on a MAbPac SCX-10 column. The use of Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of reference material monoclonal antibody charge variants using a 30 minute gradient.
Market: BioPharma
Keywords: Biotherapeutics, MAbPac SCX-10 Column, Vanquish Flex UHPLC System, Biosimilars, pH gradient buffer, charge variant analysis, NISTmAb, reference material 8671, Humanized IgG1K, NIBRT, Bioprocessing Research
Matrix: NIST standard
Author: Silvia Millán, Amy Farrell and Jonathan Bones
Affiliation: NIBRT, Dublin, Ireland
Status: Proof of Concept
Uploaded on 2/13/2017.

For Research Use Only. Not for use in diagnostic procedures.