The Thermo Scientific Vanquish Flex UHPLC system with UV detection is applied for the charge variants analysis of NISTmAb Humanized IgG1K monoclonal antibody using strong cation exchange-based chromatographic separation on a MAbPac SCX-10 column. The use of Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of reference material monoclonal antibody charge variants using a 30 minute gradient.

This application provides a fast and accurate way for biopharmaceutical chemists to monitor PEGylation reactions by using HPLC with complementary UV/Vis and charged aerosol detection.Separation on the MAbPac RP resolves PEGylation reagents, unreacted IgG, and PEGylated IgG products that elute as a broad hump.

O-glycans released by reductive ß-elimination to preserve structural integrity can be quantitatively measured by a simple direct method that minimizes sample manipulation and cleanup. Released glycans are separated with high resolution by charge, size and polarity on a mixed mode column employing both ion exchange and HILIC separation modes, using a simple, mass spectrometry compatible mobile phase.<enter final 90 characters of description>

Cationic Lipids are used as a transfection agent in biotechnological products created in the siRNA market space. As part of the lipid-based delivery system for these bio-therapeutic agents, their quality needs to be determined. DDAB, dimethyldioctadecylammonium bromide, is such a lipid, and using the Vanquish UHPLC with the universal Corona charged aerosol detector, many impurities were separated and can be quantified.

Cationic Lipids are used as a transfection agent in biotechnological products created in the siRNA market space. As part of the lipid-based delivery system for these bio-therapeutic agents, their quality needs to be determined. DC-Cholesterol, 3ß-[N-(Dimethylaminoethane)carbamoyl]cholesterol, is such a lipid, and using the Vanquish UHPLC with the universal Corona charged aerosol detector, many impurities were separated and can be quantified.<enter final 90 characters of description>