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Label-Free Profiling of mAb O-linked Glycans by HPLC with Charged Aerosol Detection Be the first to rate this application


O-glycans released by reductive ß-elimination to preserve structural integrity can be quantitatively measured by a simple direct method that minimizes sample manipulation and cleanup. Released glycans are separated with high resolution by charge, size and polarity on a mixed mode column employing both ion exchange and HILIC separation modes, using a simple, mass spectrometry compatible mobile phase.<enter final 90 characters of description>
Market: BioPharma
Keywords: biosimilars, glycan profiling, glycoprotein, direct detection, reductive beta elimination
Matrix: Protein solution
Author: Dave Thomas
Affiliation: Thermo Fisher Scientific
Uploaded on 3/25/2016.

For Research Use Only. Not for use in diagnostic procedures.