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Label-Free Profiling of mAb O-linked Glycans by HPLC with Charged Aerosol Detection
Description
O-glycans released by reductive ß-elimination to preserve structural integrity can be quantitatively measured by a simple direct method that minimizes sample manipulation and cleanup. Released glycans are separated with high resolution by charge, size and polarity on a mixed mode column employing both ion exchange and HILIC separation modes, using a simple, mass spectrometry compatible mobile phase.<enter final 90 characters of description>| Market: | BioPharma |
| Keywords: | biosimilars, glycan profiling, glycoprotein, direct detection, reductive beta elimination |
| Matrix: | Protein solution |
| Author: | Dave Thomas |
| Affiliation: | Thermo Fisher Scientific |
For Research Use Only. Not for use in diagnostic procedures.
