Label-Free Profiling of mAb O-linked Glycans by HPLC with Charged Aerosol Detection
DescriptionO-glycans released by reductive ß-elimination to preserve structural integrity can be quantitatively measured by a simple direct method that minimizes sample manipulation and cleanup. Released glycans are separated with high resolution by charge, size and polarity on a mixed mode column employing both ion exchange and HILIC separation modes, using a simple, mass spectrometry compatible mobile phase.<enter final 90 characters of description>
|Keywords:||biosimilars, glycan profiling, glycoprotein, direct detection, reductive beta elimination|
|Affiliation:||Thermo Fisher Scientific|
For Research Use Only. Not for use in diagnostic procedures.