Find methods for your needs
Refine by Feature
Displaying 1-5 of 40 results for Tag: Protein
High-precision, automated peptide mapping of proteinsInstrument Type: UHPLC
The biopharmaceutical industry continues to develop protein-based biotherapeutics in increasing numbers. Due to their complexity and biotechnological production, there are many attributes that need to be analyzed to guarantee their safety and efficacy. Peptide mapping is used to measure several critical quality attributes (CQA) required for the characterization of any biotherapeutic protein. The analysis is used to confirm that the correct sequence has been expressed for the protein and to check for post-translational and chemical modifications.
A pre-concentration and on-line solid phase extraction setup for the LC-MS analysis of therapeutic protein mixturesInstrument Type: LCMS
This study shows a fully automated UHPLC setup applying a simple and efficient online solid phase extraction method. Showcase assay performance by using a column cartridge for sample trapping and a Thermo Scientific™ MAbPac™ RP column for separation with water/acetonitrile-based gradients. Leverage the new technologies such as the Thermo Scientific™ Vanquish™ UHPLC platform in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole Orbitrap mass spectrometer.
Accurately Monitor Protein PEGylation Reactions by HPLC-UV-CADInstrument Type: HPLC-CAD
This application provides a fast and accurate way for biopharmaceutical chemists to monitor PEGylation reactions by using HPLC with complementary UV/Vis and charged aerosol detection.Separation on the MAbPac RP resolves PEGylation reagents, unreacted IgG, and PEGylated IgG products that elute as a broad hump.
Top Down Milk Protein Identification and Relative Quantification by Q Exactive Mass SpectrometerInstrument Type: LCMSMS
Whey proteins, most notably alpha-lactalbumin and beta-lactoglobulin, are currently being investigated for their potential positive health benefits. Whey proteins are a substantial percentage of human milk (~60%) while such proteins are less abundant in cow milk (~20%). We describe a method for the identification of the various forms of caseins and whey proteins as a first step and then intact protein-based method for determination of the whey vs. casein protein ratio.
Label-Free Profiling of mAb O-linked Glycans by HPLC with Charged Aerosol DetectionInstrument Type: HPLC-CAD
O-glycans released by reductive ß-elimination to preserve structural integrity can be quantitatively measured by a simple direct method that minimizes sample manipulation and cleanup. Released glycans are separated with high resolution by charge, size and polarity on a mixed mode column employing both ion exchange and HILIC separation modes, using a simple, mass spectrometry compatible mobile phase.<enter final 90 characters of description>