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A pre-concentration and on-line solid phase extraction setup for the LC-MS analysis of therapeutic protein mixtures Be the first to rate this application


This study shows a fully automated UHPLC setup applying a simple and efficient online solid phase extraction method. Showcase assay performance by using a column cartridge for sample trapping and a Thermo Scientific™ MAbPac™ RP column for separation with water/acetonitrile-based gradients. Leverage the new technologies such as the Thermo Scientific™ Vanquish™ UHPLC platform in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole Orbitrap mass spectrometer.
Market: BioPharma
Keywords: Intact protein, Monoclonal antibody, Preconcentration, Vanquish, Biopharmaceutical, mass spectrometry, MAbPac, mAb, Q Exactive, Biomolecules, MSPac, Reversed phase, trap and elute, desalt
Matrix: Drug product
Author: Martin Samonig, Sabrina Patzelt, Martin Rühl, and Remco Swart
Affiliation: Thermo Fisher Scientific, Germering, Germany
Uploaded on 2/2/2017.

For Research Use Only. Not for use in diagnostic procedures.