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Displaying 1-5 of 59 results for Tag: LCMS

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A pre-concentration and on-line solid phase extraction setup for the LC-MS analysis of therapeutic protein mixtures

Instrument Type: LCMS

This study shows a fully automated UHPLC setup applying a simple and efficient online solid phase extraction method. Showcase assay performance by using a column cartridge for sample trapping and a Thermo Scientific™ MAbPac™ RP column for separation with water/acetonitrile-based gradients. Leverage the new technologies such as the Thermo Scientific™ Vanquish™ UHPLC platform in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole Orbitrap mass spectrometer.

SMART Digest compared to classic in-solution digestion of rituximab for in-depth peptide mapping characterization

Instrument Type: LCMS

This study compares the newly developed Thermo Scientific™ SMART Digest™ kit to classic in-solution protein digestion methods, focusing on protein sequence coverage and identified post-translational modifications (PTMs), including deamidation, oxidation, and glycosylation. A Thermo Scientific™ Acclaim™ VANQUISH™ C18 column with conventional water/acetonitrile-based gradients and the Thermo Scientific™ Vanquish™ Flex UHPLC system were used for separation in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ mass spectrometer.

Tandem UHPLC operation for high-throughput LC-MS peptide mapping analyses

Instrument Type: LCMS

To demonstrate the use of a multi-pump UHPLC system and enable tandem analysis with two columns in parallel, addressing productivity and throughput improvement of existing LC-MS methods.

Determination of Meat Authenticity Using a Comprehensive Targeted Proteomic Strategy and High-Resolution Mass Spectrometry

Instrument Type: LCMS

We present a summary of a sensitive and robust HRAM LC-MS method using a Thermo Scientific Q Exactive mass spectrometer for the identification and detection of marker proteins in raw meat samples. Four proteotypic peptides were used to perform accurate meat speciation and authenticity. Myoglobin tryptic peptides from each species were detected with an observed m/z below 1.3 ppm. This targeted method allowed for the detection of undesired meat species down to 1% (w/w) of the entire sample.

Rapid Screening of Dietary Supplements with Direct Analysis ID-CUBE Coupled to an Exactive Mass Spectrometer

Instrument Type: LCMS

In Westernized cultures, dietary supplements have been gaining popularity in recent years. In China, traditional herbal supplements have been used for millennia. In both cases, the threat of adulteration and need for further characterization of ingredients have been driving forces behind the need for increased screening. This note presents a new approach to the rapid screening of a product marketed as an herbal weight loss aid and the fingerprint characterization of omega fatty acid dietary supplements derived from marine and flaxseed oils.