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SMART Digest compared to classic in-solution digestion of rituximab for in-depth peptide mapping characterization Be the first to rate this application

smart digest evaluation


This study compares the newly developed Thermo Scientific™ SMART Digest™ kit to classic in-solution protein digestion methods, focusing on protein sequence coverage and identified post-translational modifications (PTMs), including deamidation, oxidation, and glycosylation. A Thermo Scientific™ Acclaim™ VANQUISH™ C18 column with conventional water/acetonitrile-based gradients and the Thermo Scientific™ Vanquish™ Flex UHPLC system were used for separation in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ mass spectrometer.
Market: BioPharma
Keywords: Monoclonal antibody, Peptide mapping, Vanquish, Tryptic digest, Biopharmaceutical, mass spectrometry, mAb, Q Exactive, Biomolecules, Reversed phase, Orbitrap, SMART Digest kit, in-solution protein digestion
Matrix: Drug product
Author: Martin Samonig; Alexander Schwahn; Ken Cook; Mike Oliver; Remco Swart
Affiliation: Thermo Fisher Scientific
Uploaded on 1/13/2017.

For Research Use Only. Not for use in diagnostic procedures.