Find methods for your needs
Refine by Feature
Displaying 1-5 of 9 results for Tag: Vanquish
High resolution charge variants analysis of recombinant human IgG1K NIST mAbInstrument Type: HPLC-CAD
The Thermo Scientific Vanquish Flex UHPLC system with UV detection is applied for the charge variants analysis of NISTmAb Humanized IgG1K monoclonal antibody using strong cation exchange-based chromatographic separation on a MAbPac SCX-10 column. The use of Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of reference material monoclonal antibody charge variants using a 30 minute gradient.
A pre-concentration and on-line solid phase extraction setup for the LC-MS analysis of therapeutic protein mixturesInstrument Type: LCMS
This study shows a fully automated UHPLC setup applying a simple and efficient online solid phase extraction method. Showcase assay performance by using a column cartridge for sample trapping and a Thermo Scientific™ MAbPac™ RP column for separation with water/acetonitrile-based gradients. Leverage the new technologies such as the Thermo Scientific™ Vanquish™ UHPLC platform in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole Orbitrap mass spectrometer.
SMART Digest compared to classic in-solution digestion of rituximab for in-depth peptide mapping characterizationInstrument Type: LCMS
This study compares the newly developed Thermo Scientific™ SMART Digest™ kit to classic in-solution protein digestion methods, focusing on protein sequence coverage and identified post-translational modifications (PTMs), including deamidation, oxidation, and glycosylation. A Thermo Scientific™ Acclaim™ VANQUISH™ C18 column with conventional water/acetonitrile-based gradients and the Thermo Scientific™ Vanquish™ Flex UHPLC system were used for separation in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ mass spectrometer.
Tandem UHPLC operation for high-throughput LC-MS peptide mapping analysesInstrument Type: LCMS
To demonstrate the use of a multi-pump UHPLC system and enable tandem analysis with two columns in parallel, addressing productivity and throughput improvement of existing LC-MS methods.
Rapid Separation of Eighteen Herbicides Using Advanced UHPLC and Sub-2 µm Solid Core Column TechnologiesInstrument Type: UHPLC
Environmental monitoring of herbicides used in the production of food crops is becoming more widespread due to their increased use and inevitable leaching from soil into water sources. This application shows the advantages of using the Thermo Scientific Accucore Vanquish C18 1.5 µm UHPLC column and the Vanquish UHPLC system for the separation of 18 herbicides.