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Displaying 1-3 of 3 results for Tag: Reversed phase
A pre-concentration and on-line solid phase extraction setup for the LC-MS analysis of therapeutic protein mixtures
Instrument Type: LCMSThis study shows a fully automated UHPLC setup applying a simple and efficient online solid phase extraction method. Showcase assay performance by using a column cartridge for sample trapping and a Thermo Scientific™ MAbPac™ RP column for separation with water/acetonitrile-based gradients. Leverage the new technologies such as the Thermo Scientific™ Vanquish™ UHPLC platform in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole Orbitrap mass spectrometer.
SMART Digest compared to classic in-solution digestion of rituximab for in-depth peptide mapping characterization
Instrument Type: LCMSThis study compares the newly developed Thermo Scientific™ SMART Digest™ kit to classic in-solution protein digestion methods, focusing on protein sequence coverage and identified post-translational modifications (PTMs), including deamidation, oxidation, and glycosylation. A Thermo Scientific™ Acclaim™ VANQUISH™ C18 column with conventional water/acetonitrile-based gradients and the Thermo Scientific™ Vanquish™ Flex UHPLC system were used for separation in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ mass spectrometer.
Fast Online Desalting of mAbs Using a Reversed-Phase Desalting Cartridge for LC-MS Analysis
Instrument Type: LCMSThis collateral describes a LCMS method for high throughput intact antibody characterization, capable of delivering the intact mass and the relative glycoform abundance of a mAb within 5 minutes.The desalting method optimization was conducted on a 2.1 × 10 mm Thermo Scientific™ MSPac™ DS-10 cartridge using conventional water/acetonitrile-based separations on the Thermo Scientific™ Vanquish™ UHPLC system and MS detection using the Thermo Scientific™ Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ mass spectrometer.