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Fast Online Desalting of mAbs Using a Reversed-Phase Desalting Cartridge for LC-MS Analysis
Description
This collateral describes a LCMS method for high throughput intact antibody characterization, capable of delivering the intact mass and the relative glycoform abundance of a mAb within 5 minutes.The desalting method optimization was conducted on a 2.1 × 10 mm Thermo Scientific™ MSPac™ DS-10 cartridge using conventional water/acetonitrile-based separations on the Thermo Scientific™ Vanquish™ UHPLC system and MS detection using the Thermo Scientific™ Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ mass spectrometer.| Market: | BioPharma |
| Keywords: | Glycosylation, Intact protein, Monoclonal antibody, Vanquish, Biopharmaceutical, mass spectrometry, mAb, Q Exactive, Biomolecules, Desalting, MSPac, Reversed phase |
| Matrix: | Drug product |
| Author: | Martin Samonig, Shane Bechler, Kelly Flook, Remco Swart |
| Affiliation: | Thermo Fisher Scientific |
For Research Use Only. Not for use in diagnostic procedures.
