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Displaying 1-5 of 6 results for Tag: mass spectrometry

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A pre-concentration and on-line solid phase extraction setup for the LC-MS analysis of therapeutic protein mixtures

Instrument Type: LCMS

This study shows a fully automated UHPLC setup applying a simple and efficient online solid phase extraction method. Showcase assay performance by using a column cartridge for sample trapping and a Thermo Scientific™ MAbPac™ RP column for separation with water/acetonitrile-based gradients. Leverage the new technologies such as the Thermo Scientific™ Vanquish™ UHPLC platform in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole Orbitrap mass spectrometer.

SMART Digest compared to classic in-solution digestion of rituximab for in-depth peptide mapping characterization

Instrument Type: LCMS

This study compares the newly developed Thermo Scientific™ SMART Digest™ kit to classic in-solution protein digestion methods, focusing on protein sequence coverage and identified post-translational modifications (PTMs), including deamidation, oxidation, and glycosylation. A Thermo Scientific™ Acclaim™ VANQUISH™ C18 column with conventional water/acetonitrile-based gradients and the Thermo Scientific™ Vanquish™ Flex UHPLC system were used for separation in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ mass spectrometer.

Evaluation of Prelude SPLC and TSQ Endura Mass Spectrometer in Research Analysis of Testosterone in Human Serum

Instrument Type: LCMSMS

The analysis of testosterone in human serum for clinical research is currently performed using many different sample preparation procedures. In this note, we evaluated a Prelude SPLC system coupled to a TSQ Endura MS (Figure 1) in the analysis of testosterone using two sample preparation methods: online sample cleanup and offline sample cleanup. Each method uses specific chromatography, but both share the same mass spectrometer settings and parameters.

Fast Online Desalting of mAbs Using a Reversed-Phase Desalting Cartridge for LC-MS Analysis

Instrument Type: LCMS

This collateral describes a LCMS method for high throughput intact antibody characterization, capable of delivering the intact mass and the relative glycoform abundance of a mAb within 5 minutes.The desalting method optimization was conducted on a 2.1 × 10 mm Thermo Scientific™ MSPac™ DS-10 cartridge using conventional water/acetonitrile-based separations on the Thermo Scientific™ Vanquish™ UHPLC system and MS detection using the Thermo Scientific™ Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ mass spectrometer.

AN263: Sensitive and Fast Determination of Endothall in Saline Water Sample by IC-MS/MS

Instrument Type: ICMS

Endothall, a widely used herbicide for both terrestrial and aquatic weeds, is a regulated contaminant for drinking water having a California Public Health Goal of 580 ppb. Here trace-level endothall in a saline water sample was determined directly without labor-intensive sample preparation by IC-MS-MS. The MS/MS instrument was operated in selected reaction monitoring (SRM) mode, requiring minimal sample cleanup and ensuring highly sensitive (low ppb) and selective quantitation. Isotope labeled glutaric acid (glutaric acid–d6) was used as an internal standard to ensure quantitation accuracy.