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Displaying 1-5 of 22 results for Tag: Monoclonal antibody

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TN72264: HPAE-PAD N-linked Oligosaccharide Profiling of IgG

Instrument Type: IC

This technical note reports an HPAE-PAD method to profile IgG N-linked oligosaccharides and N-linked high-mannose type oligosaccharides that are atypical for human polyclonal IgG but sometimes found on mAbs. This HPAE-PAD method is orthogonal to other oligosaccharide methods applied to IgG. More information on the development and application of the separation described in the technical note can be found in Rohrer, et al. as referenced in the document.

Separation of oxidized and deamidated impurities of Infliximab by reverse phase chromatography

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific Dionex UltiMate 3000 Bio-LC system is applied for the Reverse phase analysis of Infliximab. The separation was performed on a Thermo Scientific MabPac RP column with UV detection at 214 nm. All oxidized and deamidated impurities are well separated.

A pre-concentration and on-line solid phase extraction setup for the LC-MS analysis of therapeutic protein mixtures

Instrument Type: LCMS

This study shows a fully automated UHPLC setup applying a simple and efficient online solid phase extraction method. Showcase assay performance by using a column cartridge for sample trapping and a Thermo Scientific™ MAbPac™ RP column for separation with water/acetonitrile-based gradients. Leverage the new technologies such as the Thermo Scientific™ Vanquish™ UHPLC platform in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole Orbitrap mass spectrometer.

Ultrafast hydrophilic interaction UHPLC analysis of 2AA labelled N-glycans from monoclonal antibodies (mAbs)

Instrument Type: UHPLC

The Thermo Scientific Vanquish UHPLC system with Fluorescence detection is applied for the rapid glyco-profiling of a commercial chimeric IgG1 mAb.The separation is performed on a Thermo Scientific Accucore-150-Amide HILIC column in under 2.5 minutes. The developed methodology offers an excellent analytical platform for ultrafast glycan profile comparison of different batches​ of these mAbs.

SMART Digest compared to classic in-solution digestion of rituximab for in-depth peptide mapping characterization

Instrument Type: LCMS

This study compares the newly developed Thermo Scientific™ SMART Digest™ kit to classic in-solution protein digestion methods, focusing on protein sequence coverage and identified post-translational modifications (PTMs), including deamidation, oxidation, and glycosylation. A Thermo Scientific™ Acclaim™ VANQUISH™ C18 column with conventional water/acetonitrile-based gradients and the Thermo Scientific™ Vanquish™ Flex UHPLC system were used for separation in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ mass spectrometer.