Ultrafast hydrophilic interaction UHPLC analysis of 2AA labelled N-glycans from monoclonal antibodies (mAbs)
DescriptionThe Thermo Scientific Vanquish UHPLC system with Fluorescence detection is applied for the rapid glyco-profiling of a commercial chimeric IgG1 mAb.The separation is performed on a Thermo Scientific Accucore-150-Amide HILIC column in under 2.5 minutes. The developed methodology offers an excellent analytical platform for ultrafast glycan profile comparison of different batches of these mAbs.
|Keywords:||Biotherapeutics, Glycosylation, IgG, N-glycans, Rituximab, Glycoprofiling, Monoclonal Antibodies (mAbs),, Accucore-Amide-HILIC, NIBRT, Bioprocessing Research|
|Matrix:||Rituximab drug product 10 mg/mL (MabThera®)|
|Author:||Silvia Millán, Stefan Mittermayr, Amy Farrell and Jonathan Bones|
|Affiliation:||NIBRT, Dublin, Ireland|
|Status:||Proof of Concept|
For Research Use Only. Not for use in diagnostic procedures.