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Ultrafast hydrophilic interaction UHPLC analysis of 2AA labelled N-glycans from monoclonal antibodies (mAbs) Be the first to rate this application


The Thermo Scientific Vanquish UHPLC system with Fluorescence detection is applied for the rapid glyco-profiling of a commercial chimeric IgG1 mAb.The separation is performed on a Thermo Scientific Accucore-150-Amide HILIC column in under 2.5 minutes. The developed methodology offers an excellent analytical platform for ultrafast glycan profile comparison of different batches​ of these mAbs.
Market: BioPharma
Keywords: Biotherapeutics, Glycosylation, IgG, N-glycans, Rituximab, Glycoprofiling, Monoclonal Antibodies (mAbs),, Accucore-Amide-HILIC, NIBRT, Bioprocessing Research
Matrix: Rituximab drug product 10 mg/mL (MabThera®)
Author: Silvia Millán, Stefan Mittermayr, Amy Farrell and Jonathan Bones
Affiliation: NIBRT, Dublin, Ireland
Status: Proof of Concept
Uploaded on 1/17/2017.

For Research Use Only. Not for use in diagnostic procedures.