To demonstrate how the use of alternate UHPLC stationary phase selectivities can facilitate method speed up and as a result this sample throughput was tripled compared to the legacy methods with an associated five-fold reduction in cost per sample for optimized method

Five-fold increase in method throughput compared to original method (fifty samples/hour). Associated 10-fold reduction in cost per sample through reduced mobile phase consumption and waste generation. Additional reduced method complexity from easy-to-prepare mobile phase

Improved separation of closely related glucocorticoids with reduced method complexity High-throughput analysis possible through a reduced complexity, rapid, two-minute isocratic method Associated reduction in cost per sample through reduced mobile phase consumption and waste generation

To demonstrate practical approaches that can be used to significantly improve throughput of the cephradine USP assay monograph keeping to the spirit of USP-NF Chapter <621> guidelines while maintaining USP quality acceptance criteria.

To demonstrate practical approaches that can be used to significantly improve throughput of the ibuprofen USP assay monograph keeping to the spirit o USP-NF Chapter <621> guidelines while maintaining USP quality acceptance criteria. To then take this optimized assay monograph and reduce analysis time even further.