TN72264: HPAE-PAD N-linked Oligosaccharide Profiling of IgG
DescriptionThis technical note reports an HPAE-PAD method to profile IgG N-linked oligosaccharides and N-linked high-mannose type oligosaccharides that are atypical for human polyclonal IgG but sometimes found on mAbs. This HPAE-PAD method is orthogonal to other oligosaccharide methods applied to IgG. More information on the development and application of the separation described in the technical note can be found in Rohrer, et al. as referenced in the document.
|Keywords:||Biotherapeutics, Electrochemical detection, Monoclonal antibody, recombinant glycoprotein, glycan analysis, CarboPac PA200, mAb, Ion chromatograhy, Glycan, TN71, TN110|
|Author:||Sachin Patil, Deanna Hurum, Lipika Basumallick, and Jeff Rohrer|
|Affiliation:||Thermo Fisher Scientific, Sunnyvale, CA 94085|
For Research Use Only. Not for use in diagnostic procedures.