TN72264: HPAE-PAD N-linked Oligosaccharide Profiling of IgG

Instrument Type: IC

This technical note reports an HPAE-PAD method to profile IgG N-linked oligosaccharides and N-linked high-mannose type oligosaccharides that are atypical for human polyclonal IgG but sometimes found on mAbs. This HPAE-PAD method is orthogonal to other oligosaccharide methods applied to IgG. More information on the development and application of the separation described in the technical note can be found in Rohrer, et al. as referenced in the document.

AU141: Improved Long-Term Stability of N-Acetylneuraminic Acid and N-Glycolylneuraminic Acid Peak Area Responses Using Waveform A, a Quadruple Potential Waveform

Instrument Type: IC

In TN41, “Analysis of Sialic Acids Using High-Performance Anion-Exchange Chromatography”, we showed that the sialic acid content of a glycoprotein could be accurately and reproducibly determined by HPAE-PAD. That Note showed the separation of Neu5Ac, Neu5Gc, and the internal standard 3-deoxy-D-glycero-D-galacto-2-nonulosonic acid. In this Application Update we show that Waveform A offers 48h peak area reproducibility (peak area RSDs <5%) for sialic acids analyzed by HPAE-PAD similar to that reported in TN41, and improved reproducibility of peak area response with long-term (> 48 h) use.