Rate
Ask the Expert
Suggest an Application
Add to Favorites
CreateParts List
Tell a Friend
PrintFind methods for your needs
AU141: Improved Long-Term Stability of N-Acetylneuraminic Acid and N-Glycolylneuraminic Acid Peak Area Responses Using Waveform A, a Quadruple Potential Waveform
Description
In TN41, “Analysis of Sialic Acids Using High-Performance Anion-Exchange Chromatography”, we showed that the sialic acid content of a glycoprotein could be accurately and reproducibly determined by HPAE-PAD. That Note showed the separation of Neu5Ac, Neu5Gc, and the internal standard 3-deoxy-D-glycero-D-galacto-2-nonulosonic acid. In this Application Update we show that Waveform A offers 48h peak area reproducibility (peak area RSDs <5%) for sialic acids analyzed by HPAE-PAD similar to that reported in TN41, and improved reproducibility of peak area response with long-term (> 48 h) use.| Market: | BioPharma |
| Keywords: | carbohydrate, Electrochemical detection, glycoprotein, KDN, KDO, NANA, Neu5Ac, Neu5Gc, NGNA, recombinant glycoprotein, sialic acid, sugar acid, PAD, HPAE-PAD, TN71, TN 41, AU 141, TN41, AU 180, TN 20, TN 70671, TN20, TN70671, AU141, AU180, AU 181, AU181 |
| Matrix: | Glycoprotein |
| Author: | Jeffrey Rohrer |
| Affiliation: | Thermo Fisher Scientific, Sunnyvale, CA USA |
For Research Use Only. Not for use in diagnostic procedures.
