AN253: HPAE-PAD Determination of Infant Formula Sialic Acid

Instrument Type: IC

Sialic acids in infant formulas are accurately determined by HPAE-PAD using the Dionex CarboPac PA20 column following acid hydrolysis and maltodextrin removal using one of two sample-preparation methods. HPAE-PAD provides reliable determination of sialic acids in acid hydrolyzed infant formula samples without sample derivatization. This method may be used to quantify sialic acids in formulas that have been enriched with sialic acids.

TN41: Analysis of Sialic Acids Using High-Performance Anion-Exchange Chromatography

Instrument Type: IC

Linearity, detection limits, reproducibility, and accuracy of HPAE-PAD analysis of Neu5Ac and Neu5Gc are evaluated in this Technical Note. A variety of ways to separate Neu5Ac, Neu5Gc, and KDN (3-deoxy-D-glycero-D-galacto-2-nonulosonic acid), an internal standard, using the CarboPac PA10 column as well as methods to release sialic acids from glycoproteins are included in this Note. Readers should also see AU141, AU180, and AU181.

AU141: Improved Long-Term Stability of N-Acetylneuraminic Acid and N-Glycolylneuraminic Acid Peak Area Responses Using Waveform A, a Quadruple Potential Waveform

Instrument Type: IC

In TN41, “Analysis of Sialic Acids Using High-Performance Anion-Exchange Chromatography”, we showed that the sialic acid content of a glycoprotein could be accurately and reproducibly determined by HPAE-PAD. That Note showed the separation of Neu5Ac, Neu5Gc, and the internal standard 3-deoxy-D-glycero-D-galacto-2-nonulosonic acid. In this Application Update we show that Waveform A offers 48h peak area reproducibility (peak area RSDs <5%) for sialic acids analyzed by HPAE-PAD similar to that reported in TN41, and improved reproducibility of peak area response with long-term (> 48 h) use.