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TN41: Analysis of Sialic Acids Using High-Performance Anion-Exchange Chromatography
Description
Linearity, detection limits, reproducibility, and accuracy of HPAE-PAD analysis of Neu5Ac and Neu5Gc are evaluated in this Technical Note. A variety of ways to separate Neu5Ac, Neu5Gc, and KDN (3-deoxy-D-glycero-D-galacto-2-nonulosonic acid), an internal standard, using the CarboPac PA10 column as well as methods to release sialic acids from glycoproteins are included in this Note. Readers should also see AU141, AU180, and AU181.| Market: | BioPharma |
| Keywords: | carbohydrate, Dionex, Electrochemical detection, NANA, Neu5Ac, Neu5Gc, Neuraminidase, NGNA, sialic acid, CarboPac PA10, PAD, HPAE-PAD, TN71, TN 71, TN 41, AU 141, TN41, AU 180, TN 20, TN 70671, TN20, TN70671, AU141, AU180, AU 181, AU181 |
| Matrix: | Glycoprotein |
| Author: | Jeffrey Rohrer |
| Affiliation: | Thermo Fisher Scientific, Sunnyvale, CA USA |
For Research Use Only. Not for use in diagnostic procedures.
