TN53: Determination of Glycoprotein Monosaccharide Composition by HPAE-PAD Using On-Line Electrolytically Generated Eluents

Instrument Type: IC

This is the first document showing the use of eluent generation for HPAE-PAD monosaccharide analysis of glycoproteins. Those interested in an updated version should see TN40. This record is provided for those with an EG40 and a Dionex CarboPac PA10 column that would like to do this application. This document also has useful sample preparation information for HPAE-PAD glycorprotein monosaccharide analysis.

AN117: Quantification of Carbohydrates and Glycols in Pharmaceuticals - Small Sugars

Instrument Type: IC

This application note describes the use of three different anion-exchange columns with amperometric detection to analyze common simple sugars, sugar alcohols, and glycols in pharmaceutical formulations. This record describes a method using the CarboPac PA10 column to determine some of the target carbohydrates. The reader is advised to review TN21 for updated waveform information.

TN41: Analysis of Sialic Acids Using High-Performance Anion-Exchange Chromatography

Instrument Type: IC

Linearity, detection limits, reproducibility, and accuracy of HPAE-PAD analysis of Neu5Ac and Neu5Gc are evaluated in this Technical Note. A variety of ways to separate Neu5Ac, Neu5Gc, and KDN (3-deoxy-D-glycero-D-galacto-2-nonulosonic acid), an internal standard, using the CarboPac PA10 column as well as methods to release sialic acids from glycoproteins are included in this Note. Readers should also see AU141, AU180, and AU181.