This work describes an HPAE-PAD method for monosaccharide composition analysis using manually prepared eluent. Monosaccharide analysis using electrolytically generated eluent has been described before. Here, three commercially available proteins, IgG, fetuin, and alpha-1-acid glycoprotein, were individually subjected to two hydrolysis conditions using 1)HCl, for the amino sugars galactosamine and glucosamine, and 2)TFA, for the neutral sugars mannose, glucose, and galactose. Results for method linearity, robustness, and accuracy are presented here. An ICS-6000 can be used for this application.

O-glycans released by reductive ß-elimination to preserve structural integrity can be quantitatively measured by a simple direct method that minimizes sample manipulation and cleanup. Released glycans are separated with high resolution by charge, size and polarity on a mixed mode column employing both ion exchange and HILIC separation modes, using a simple, mass spectrometry compatible mobile phase.<enter final 90 characters of description>

This technical note describes the use of exoglycosidases to obtain structural information about N-linked oligosaccharides commonly found in mammalian glycoproteins. HPAE-PAD is used to confirm the identity of the released monosaccharides, as well as the remaining di-, tri,- or oligosaccharides. Empirical rules used to predict oligosaccharide elution order are included in the last section of this technical note.

This technical note describes the use of exoglycosidases to obtain structural information about N-linked oligosaccharides commonly found in mammalian glycoproteins. HPAE-PAD is used to confirm the identity of the released monosaccharides, as well as the remaining di-, tri,- or oligosaccharides. Empirical rules used to predict oligosaccharide elution order are included in the last section of this technical note.

This technical note describes the use of exoglycosidases to obtain structural information about N-linked oligosaccharides commonly found in mammalian glycoproteins. HPAE-PAD is used to confirm the identity of the released monosaccharides, as well as the remaining di-, tri,- or oligosaccharides. Empirical rules used to predict oligosaccharide elution order are included in the last section of this technical note.