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TN36: Analysis of Exoglycosidase Digestions of N-Linked Oligosaccharides Using HPAE-PAD (Part A)
Description
This technical note describes the use of exoglycosidases to obtain structural information about N-linked oligosaccharides commonly found in mammalian glycoproteins. HPAE-PAD is used to confirm the identity of the released monosaccharides, as well as the remaining di-, tri,- or oligosaccharides. Empirical rules used to predict oligosaccharide elution order are included in the last section of this technical note.| Market: | BioPharma |
| Keywords: | glycoprotein, HPAE-PAD, Exoglycosidase, N-linked oligosaccharides, TN71, TN 20, TN 70671, TN20, TN70671, TN 36 |
| Matrix: | Water |
| Affiliation: | Thermo Fisher Scientific, Sunnyvale, CA USA |
For Research Use Only. Not for use in diagnostic procedures.
