TN36: Analysis of Exoglycosidase Digestions of N-Linked Oligosaccharides Using HPAE-PAD (Part A)

Instrument Type: IC

This technical note describes the use of exoglycosidases to obtain structural information about N-linked oligosaccharides commonly found in mammalian glycoproteins. HPAE-PAD is used to confirm the identity of the released monosaccharides, as well as the remaining di-, tri,- or oligosaccharides. Empirical rules used to predict oligosaccharide elution order are included in the last section of this technical note.