TN41: Analysis of Sialic Acids Using High-Performance Anion-Exchange Chromatography

Instrument Type: IC

Linearity, detection limits, reproducibility, and accuracy of HPAE-PAD analysis of Neu5Ac and Neu5Gc are evaluated in this Technical Note. A variety of ways to separate Neu5Ac, Neu5Gc, and KDN (3-deoxy-D-glycero-D-galacto-2-nonulosonic acid), an internal standard, using the CarboPac PA10 column as well as methods to release sialic acids from glycoproteins are included in this Note. Readers should also see AU141, AU180, and AU181.

AN215: Separation of Asparagine-Linked (N-Linked) Oligosaccharides from Human Polyclonal IgG

Instrument Type: IC

This application note shows how the PA200 improves N-linked oligosaccharide separations. The PA200 is used to profile N-linked oligosaccharides released from human polyclonal IgG by the enzyme PNGase F or endoglycosidase H (Endo H). This note also shows how subsequent exoglycosidase digestions can be used to assist in understanding and identifying oligosaccharide structure.