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AN215: Separation of Asparagine-Linked (N-Linked) Oligosaccharides from Human Polyclonal IgG Be the first to rate this application

an215 separation asparaginelinked nlinked oligosaccharides from human polyclonal igg

Description

This application note shows how the PA200 improves N-linked oligosaccharide separations. The PA200 is used to profile N-linked oligosaccharides released from human polyclonal IgG by the enzyme PNGase F or endoglycosidase H (Endo H). This note also shows how subsequent exoglycosidase digestions can be used to assist in understanding and identifying oligosaccharide structure.
Market: Pharma
Keywords: AN 215, Beta-galactosidase, CarboPac PA1, Disaccharides, Endo H, Endoglycosidase H, fetuin, Glycoproteins, Human Polyclonal IgG, Monosaccharides, N-acetylneuraminic acid, Neuraminidase, N-glycolylneuraminic acid, Oligosaccharides, PNGase F, Polysaccharides, Sialic acids, Sugar acids, Sugar alcohols, Sugar nucleotides, Sugar phosphates
Matrix: Water
Uploaded on 2/4/2015.

For Research Use Only. Not for use in diagnostic procedures.