AU141: Improved Long-Term Stability of N-Acetylneuraminic Acid and N-Glycolylneuraminic Acid Peak Area Responses Using Waveform A, a Quadruple Potential Waveform

Instrument Type: IC

In TN41, “Analysis of Sialic Acids Using High-Performance Anion-Exchange Chromatography”, we showed that the sialic acid content of a glycoprotein could be accurately and reproducibly determined by HPAE-PAD. That Note showed the separation of Neu5Ac, Neu5Gc, and the internal standard 3-deoxy-D-glycero-D-galacto-2-nonulosonic acid. In this Application Update we show that Waveform A offers 48h peak area reproducibility (peak area RSDs <5%) for sialic acids analyzed by HPAE-PAD similar to that reported in TN41, and improved reproducibility of peak area response with long-term (> 48 h) use.

AN215: Separation of Asparagine-Linked (N-Linked) Oligosaccharides from Human Polyclonal IgG

Instrument Type: IC

This application note shows how the PA200 improves N-linked oligosaccharide separations. The PA200 is used to profile N-linked oligosaccharides released from human polyclonal IgG by the enzyme PNGase F or endoglycosidase H (Endo H). This note also shows how subsequent exoglycosidase digestions can be used to assist in understanding and identifying oligosaccharide structure.