A fast separation of 2-AB labelled N-Glycans from polyclonal human serum IgG

Instrument Type: UHPLC

The Thermo Scientific Vanquish H UHPLC system is applied for the rapid determination of 2-AB (2-aminobenzamide) labelled N-glycans released from human serum IgG in less than 15 minutes using a 50 mm Thermo Scientific Accucore 150-Amide-HILIC column with fluorescence detection. The method is able to determine N-Glycans present at the asparagine 297 residue in the CH2 domain of the Fc region of IgG that play a crucial role in modulating the structural stability and functional activity relationship of the antibody.

AN215: Separation of Asparagine-Linked (N-Linked) Oligosaccharides from Human Polyclonal IgG

Instrument Type: IC

This application note shows how the PA200 improves N-linked oligosaccharide separations. The PA200 is used to profile N-linked oligosaccharides released from human polyclonal IgG by the enzyme PNGase F or endoglycosidase H (Endo H). This note also shows how subsequent exoglycosidase digestions can be used to assist in understanding and identifying oligosaccharide structure.

Excellent separation of human IgG glycans on a Thermo Scientific Accucore 150-Amide-HILIC HPLC column

Instrument Type: HPLC

Human IgG glycans were separated on a Thermo Scientific Accucore 150-Amide-HILIC HPLC column using 2-AB derivatization and fluorescence detection.