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Displaying 1-5 of 16 results for Tag: Sugar alcohol

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Determination of Mannitol and Protein by 2D-HPLC

Instrument Type: HPLC-CAD

Many biopharmaceutical formulations use carbohydrates to enhance the stability of proteins. This method is an example of a "heart-cut" 2D HPLC method, separating the carbohydrate analyte from the protein. The method uses two charge aerosol detectors, as an example, but one can be substituted by UV. Calibration for the Mannitol is from 15.6 - 2000 ng o.c. If a second Corona Veo SD is used for the Protein, it uses the parameters described as "Veo SD".

Determination of Sorbitol and Protein by 2D-HPLC

Instrument Type: HPLC-CAD

In bioformulations containing a protein and a carbohydrates, carbohydrates are used to assist in the stabilization of the proteins in solution, and are used in different amounts, depending on the protein and the product requirements. Sorbitol, a sugar alcohol is transferred to and analysed by HILIC and CAD after it is eluted from the reverse-phase column. Both sorbitol and the protein are analysed in this 2D-HPLC method.

Direct Determination of Unlabelled Carbohydrates, used in Biotech Formulations by HPLC-CAD

Instrument Type: HPLC-CAD

In many of the vaccines and other formulations that are used in the prevention and treatment of many conditions and illnesses contain carbohydrates, as a part of their formulation. Six carbohydrates were identified as important components in biotechnological formulations: sorbitol, mannitol, sucrose, lactose, maltose, and trehalose. These are separated by HILIC-HPLC and detected by the universal Corona charged aerosol detector.

AN122 (a): The Determination of Carbohydrates, Alcohols, and Glycols in Fermentation Broths

Instrument Type: IC

This Application Note describes the use of two different anion-exchange columns with amperometric detection to analyze simple sugars, sugar alcohols, alcohols, and glycols in yeast and bacterial fermentation broths. This record describes the CarboPac MA1 column method. The yeast Saccharomyces cerevisiae in Yeast Extract-Peptone- Dextrose (YPD) broth and the bacteria Escherichia coli (E. coli) in Luria-Bertani (LB) broth are common eukaryotic and prokaryotic fermentation systems, respectively.

AN117: Quantification of Carbohydrates andGlycols in Pharmaceuticals - Alditols and Small Carbohydrates Method

Instrument Type: IC

Two oral, over-the-counter medications were evaluated as representative pharmaceutical products. A cough suppressant and a multisymptom cold/flu medication were chosen because they contain a complex mixture of simple sugars, glycols,and sugar alcohols. These carbohydrates and glycols are also commonly found in other medications. This method uses the CarboPac MA1 column to retain and separate the target analytes.