Rate
Ask the Expert
Suggest an Application
Add to Favorites
CreateParts List
Tell a Friend
PrintFind methods for your needs
AN122 (a): The Determination of Carbohydrates, Alcohols, and Glycols in Fermentation Broths
Description
This Application Note describes the use of two different anion-exchange columns with amperometric detection to analyze simple sugars, sugar alcohols, alcohols, and glycols in yeast and bacterial fermentation broths. This record describes the CarboPac MA1 column method. The yeast Saccharomyces cerevisiae in Yeast Extract-Peptone-Dextrose (YPD) broth and the bacteria Escherichia coli (E. coli) in Luria-Bertani (LB) broth are common eukaryotic and prokaryotic fermentation systems, respectively.| Market: | BioPharma |
| Keywords: | arabinose, CarboPac MA1, Electrochemical detection, Ethanol, Glucose, Methanol, rhamnose, ribose, Glycerol, Maltose, Sorbitol, cell culture, fermentation broth, fermentation media, Lactose, Raffinose, Sucrose, mannitol, PAD, galactose, HPAE-PAD, TN21, TN71, Arabitol, Erythritol, galactitol, 2-3-Butanediol, YPD, TN 71, TN 20, TN 70671, TN20, TN70671, butanediol, TN 21, Saccharomyces cerevisiae, E. coli, Escherichia coli, S. cerevisiae, LB broth, Luria-Bertani broth, yeast extract-peptone-dextrose broth |
| Matrix: | Fermentation media |
| Author: | Valoran Hanko and Jeff Rohrer |
| Affiliation: | Thermo Fisher Scientific, Sunnyvale, CA USA |
For Research Use Only. Not for use in diagnostic procedures.
