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AN122 (a): The Determination of Carbohydrates, Alcohols, and Glycols in Fermentation Broths Be the first to rate this application

carbohydrates fermentation broths


This Application Note describes the use of two different anion-exchange columns with amperometric detection to analyze simple sugars, sugar alcohols, alcohols, and glycols in yeast and bacterial fermentation broths. This record describes the CarboPac MA1 column method. The yeast Saccharomyces cerevisiae in Yeast Extract-Peptone- Dextrose (YPD) broth and the bacteria Escherichia coli (E. coli) in Luria-Bertani (LB) broth are common eukaryotic and prokaryotic fermentation systems, respectively.
Market: BioPharma
Keywords: arabinose, CarboPac MA1, Electrochemical detection, Ethanol, Glucose, Methanol, rhamnose, ribose, Glycerol, Maltose, Sorbitol, cell culture, fermentation broth, fermentation media, Lactose, Raffinose, Sucrose, mannitol, PAD, galactose, HPAE-PAD, TN21, TN71, Arabitol, Erythritol, galactitol, 2-3-Butanediol
Matrix: Fermentation media
Author: Valoran Hanko and Jeff Rohrer
Affiliation: Thermo Fisher Scientific
Uploaded on 5/18/2015.

For Research Use Only. Not for use in diagnostic procedures.