This record demonstrates the determinations of glucose, fructose, and sucrose in a 100-fold diluted liqueur alcoholic beverage sample by HPAE-PAD. Typically samples with g/L concentrations require >10,000-fold dilutions to remain in the linear range of the very sensitive HPAE-PAD technique. However, this method demonstrated on the Integrion RFIC system, uses a 0.4 µL internal injection loop with the High Concentration Carbohydrate Analysis Kit to extend the linearity from low mg/L to g/L concentrations. A wood hydrolysate application using this technique is attached for reference.

This application note describes the analysis of residual solvents in accordance with USP method <467> using the Thermo Scientific™ TRACE™ 1310 GC, the new Thermo Scientific™ TriPlus™ 300 Headspace autosampler, and the Thermo Scientific™ Dionex™ Chromeleon™ chromatography data system (CDS) software. The method details the chromatographic system and analytical conditions to be used and addresses the acceptable limits for each solvent.

This Application Note describes the use of two different anion-exchange columns with amperometric detection to analyze simple sugars, sugar alcohols, alcohols, and glycols in yeast and bacterial fermentation broths. This record describes the CarboPac MA1 column method. The yeast Saccharomyces cerevisiae in Yeast Extract-Peptone-Dextrose (YPD) broth and the bacteria Escherichia coli (E. coli) in Luria-Bertani (LB) broth are common eukaryotic and prokaryotic fermentation systems, respectively.

This application note describes the use of ion-exchange or ion-exclusion chromatography for the determination of five classes of compounds of interest to the brewing industry, including: carbohydrates, alcohols, organic acids, inorganic anions, and inorganic cations. One of two forms of electrochemical detection is used, pulsed amperometry or conductivity detection. This part of the application note describes the alcohol analysis which uses pulsed amperometric detection. This was formerly Dionex AN46.

Goal of this application is to demonstrate the applicability of a modern, high-throughput valve-and-loop static headspace autosampler to obtain full compliance with the European Standard EN 13628-1, which specifies guidelines for the quantitative determination of residual solvents in flexible packaging by Multiple Headspace Extraction methodology. The Thermo Scientific Trace1310 GC coupled with the Triplus300 HS have been used for this purpose.