The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the affinity analysis for concentration determination of Bevacizumab Harvest Sample. The analysis was performed on a Thermo Scientific MabPac Protein A column with a UV detection at 280 nm. With a run time of 3.0 minutes, the method allows easy and fast estimation of Protein content in harvest sample of Bevacizumab. Linearity from 0.05-2.0 mg/mL with RSD of 0.5% (n=3)

This Application Note describes the use of two different anion-exchange columns with amperometric detection to analyze simple sugars, sugar alcohols, alcohols, and glycols in yeast and bacterial fermentation broths. The yeast Saccharomyces cerevisiae in Yeast Extract-Peptone-Dextrose (YPD) broth and the bacteria Escherichia coli (E. coli) in Luria-Bertani (LB) broth are common eukaryotic and prokaryotic fermentation systems, respectively. This record shows a method that is best for mono- and disaccharides.

This Application Note describes the use of two different anion-exchange columns with amperometric detection to analyze simple sugars, sugar alcohols, alcohols, and glycols in yeast and bacterial fermentation broths. This record describes the CarboPac MA1 column method. The yeast Saccharomyces cerevisiae in Yeast Extract-Peptone-Dextrose (YPD) broth and the bacteria Escherichia coli (E. coli) in Luria-Bertani (LB) broth are common eukaryotic and prokaryotic fermentation systems, respectively.

This application note describes the use of AAA-Direct to analyze common amino acids in the presence of simple sugars, sugar alcohols, alcohols, and glycols in yeast and bacterial fermentation broths. In this application note, the AminoPac PA10 anion-exchange column is used to separate amino acid and carbohydrate ingredients in cell culture and fermentation broth media. High carbohydrate concentrations can hamper the determination of some amino acids due to coelution. Also in this application note, disposable gold (Au) electrodes are evaluated for use in amino acid determination.

In this Application Note, the use of AAA-Direct for Trp determinations is investigated in proteins, peptides, and cell cultures. AAA-Direct technology provides both complete separations of all common amino acids using AminoPac PA10 and the direct detection of amino acids by integrated pulsed amperometric detection (IPAD).We present a new isocratic method designed to rapidly elute Trp while separating it from free amino acids, carbohydrates, and peptide fragments. This method reduces the run time to as short as 12 min, significantly increasing throughput compared to conventional techniques.