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Description
In this Application Note, the use of AAA-Direct for Trp determinations is investigated in proteins, peptides, and cell cultures. AAA-Direct technology provides both complete separations of all common amino acids using AminoPac PA10 and the direct detection of amino acids by integrated pulsed amperometric detection (IPAD).We present a new isocratic method designed to rapidly elute Trp while separating it from free amino acids, carbohydrates, and peptide fragments. This method reduces the run time to as short as 12 min, significantly increasing throughput compared to conventional techniques.Market: | Proteomics |
Keywords: | AAA-Certified Gold Cell, AAA-Direct, AminoPac PA10, Bacto YPD Broth, Bovine Serum Albumin (BSA), ED50A Electrochemical Detector, Escherichia coli (E. coli) cell culture, Integrated pulsed amperometry, IPAD, Luteinizing Hormone-Releasing Hormone, Trp, Tryptophan |
Matrix: | Cell cultures, Fermentation broths, Peptides, Proteins |
Author: | Valoran Hanko and Jeff Rohrer |
Affiliation: | Thermo Fisher Scientific |
For Research Use Only. Not for use in diagnostic procedures.