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AN142: Determination of Tryptophan Using AAA-Direct Be the first to rate this application

determination tryptophan

Description

In this Application Note, the use of AAA-Direct for Trp determinations is investigated in proteins, peptides, and cell cultures. AAA-Direct technology provides both complete separations of all common amino acids using AminoPac PA10 and the direct detection of amino acids by integrated pulsed amperometric detection (IPAD).We present a new isocratic method designed to rapidly elute Trp while separating it from free amino acids, carbohydrates, and peptide fragments. This method reduces the run time to as short as 12 min, significantly increasing throughput compared to conventional techniques.
Market: Proteomics
Keywords: AAA-Certified Gold Cell, AAA-Direct, AminoPac PA10, Bacto YPD Broth, Bovine Serum Albumin (BSA), ED50A Electrochemical Detector, Escherichia coli (E. coli) cell culture, Integrated pulsed amperometry, IPAD, Luteinizing Hormone-Releasing Hormone, Trp, Tryptophan
Matrix: Cell cultures, Fermentation broths, Peptides, Proteins
Author: Valoran Hanko and Jeff Rohrer
Affiliation: Thermo Fisher Scientific
Uploaded on 1/27/2015.

For Research Use Only. Not for use in diagnostic procedures.