AN142: Determination of Tryptophan Using AAA-Direct

Instrument Type: IC

In this Application Note, the use of AAA-Direct for Trp determinations is investigated in proteins, peptides, and cell cultures. AAA-Direct technology provides both complete separations of all common amino acids using AminoPac PA10 and the direct detection of amino acids by integrated pulsed amperometric detection (IPAD).We present a new isocratic method designed to rapidly elute Trp while separating it from free amino acids, carbohydrates, and peptide fragments. This method reduces the run time to as short as 12 min, significantly increasing throughput compared to conventional techniques.