AN123: Determination of Inorganic Anions and Organic Acids in Fermentation Broths

Instrument Type: IC

Fermentation broths are complex mixtures of nutrients, waste products, cells and cell debris, and desired products. Fermentation monitoring is important for many industries because carbon sources and metabolic by-products can impact the yield of the desired products. This application note describes the use of two different anion-exchange columns, IonPac AS11 and IonPac AS11-HC, with suppressed conductivity detection, to analyze common organic and inorganic anions in yeast and bacterial fermentation broths.

Improved separation of phospholipids derived from biological extracts using a Thermo Scientific Accucore RP-MS column

Instrument Type: LCMS

The Thermo Scientific HPLC system is applied for the analysis of phospholipids derived from biological extracts. The separation is performed on a Thermo Scientific Accucore C18 column, with MS detection.

AN142: Determination of Tryptophan Using AAA-Direct

Instrument Type: IC

In this Application Note, the use of AAA-Direct for Trp determinations is investigated in proteins, peptides, and cell cultures. AAA-Direct technology provides both complete separations of all common amino acids using AminoPac PA10 and the direct detection of amino acids by integrated pulsed amperometric detection (IPAD).We present a new isocratic method designed to rapidly elute Trp while separating it from free amino acids, carbohydrates, and peptide fragments. This method reduces the run time to as short as 12 min, significantly increasing throughput compared to conventional techniques.