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Concentration determination of a Bevacizumab harvest sample by using an Affinity capture Protein-A column
Description
The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the affinity analysis for concentration determination of Bevacizumab Harvest Sample. The analysis was performed on a Thermo Scientific MabPac Protein A column with a UV detection at 280 nm. With a run time of 3.0 minutes, the method allows easy and fast estimation of Protein content in harvest sample of Bevacizumab. Linearity from 0.05-2.0 mg/mL with RSD of 0.5% (n=3)| Market: | BioPharma |
| Keywords: | HPLC, UV detection, Bevacizumab, Harvest Sample, MabPac Protein A, Linearity |
| Matrix: | Cell cultures; Fermentation broths, Peptides, Proteins |
| Author: | Manish Singh |
| Affiliation: | Thermo Fisher Scientific, APAC CoE Lab, Ahmedabad, India |
For Research Use Only. Not for use in diagnostic procedures.
