The Thermo Scientific Dionex UltiMate 3000 Bio-LC system is applied for the Reverse phase analysis of Infliximab. The separation was performed on a Thermo Scientific MabPac RP column with UV detection at 214 nm. All oxidized and deamidated impurities are well separated.

The Thermo Scientific MAbPac SCX-10 column is applied to the charge variant analysis of Cetuximab. Ion exchange separation was performed using a pH gradient on a Thermo Scientific Vanquish Flex UHPLC system with UV detection at 280 nm. Use of Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of monoclonal antibody charge variants over a 30 minute gradient.

The Thermo Scientific MAbPac SCX-10 column is applied for charge variant analysis of Infliximab. Ion exchange separation was performed on a Vanquish Flex UHPLC system using a pH gradient and UV detection at 280 nm. Use of Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of monoclonal antibody charge variants over a 30 minute gradient.

Ion exchange separation is applied for charge variant analysis of Bevacizumab using a pH gradient on a Thermo Scientific MAbPac SCX-10 column. The Thermo Scientific Vanquish Flex UHPLC system with UV detection at 280 nm was used with Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of monoclonal antibody charge variants over a 30 minute gradient.

The Thermo Scientific Dionex Ultimate 3000 Bio-LC system is applied for the peptide mapping analysis of an Antibody Drug Conjugate (Kadcyla). The separation was performed on a Thermo Scientific Acclaim RSLC 120-C18 column by using a Thermo Scientific SMART digest kit at UV detection of 214 nm. With a run time of 55 minutes the method allows separation of all peptides of Kadcyla (Antibody Drug Conjugate). The SMART Digest kit provides significant improvements in reproducibility, which results in fewer sample failures, higher throughput and the ability to more easily interrogate data.