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High resolution charge variant analysis of Cetuximab by ion exchange chromatography using pH gradient buffers
Description
The Thermo Scientific MAbPac SCX-10 column is applied to the charge variant analysis of Cetuximab. Ion exchange separation was performed using a pH gradient on a Thermo Scientific Vanquish Flex UHPLC system with UV detection at 280 nm. Use of Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of monoclonal antibody charge variants over a 30 minute gradient.| Market: | BioPharma |
| Keywords: | Biotherapeutics, Monoclonal Antibodies, Cetuximab, MabPac SCX-10, pH gradient buffer, charge variant analysis, ion exchange chromatography, NIBRT, Bioprocessing Research |
| Matrix: | Drug product |
| Author: | Amy Farrell, Craig Jakes, Ken Cook and Jonathan Bones |
| Affiliation: | NIBRT, Dublin, Ireland |
For Research Use Only. Not for use in diagnostic procedures.
