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Fast and Reproducible peptide mapping of an Antibody Drug Conjugate (Kadcyla) by using a SMART digest Kit Be the first to rate this application


The Thermo Scientific Dionex Ultimate 3000 Bio-LC system is applied for the peptide mapping analysis of an Antibody Drug Conjugate (Kadcyla). The separation was performed on a Thermo Scientific Acclaim RSLC 120-C18 column by using a Thermo Scientific SMART digest kit at UV detection of 214 nm. With a run time of 55 minutes the method allows separation of all peptides of Kadcyla (Antibody Drug Conjugate). The SMART Digest kit provides significant improvements in reproducibility, which results in fewer sample failures, higher throughput and the ability to more easily interrogate data.
Market: BioPharma
Keywords: Peptide mapping, UV detection, UHPLC, Antibody Drug Conjugate, Kadcyla, Trypsin digestion, SMART Digest kit
Matrix: N/A
Author: Manish Singh
Affiliation: APAC CoE Lab, Ahmedabad, India
Uploaded on 12/16/2016.

For Research Use Only. Not for use in diagnostic procedures.