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Displaying 1-3 of 3 results for Tag: Trypsin digest
Fast and Reproducible peptide mapping of an Antibody Drug Conjugate (Kadcyla) by using a SMART digest KitInstrument Type: HPLC (Biocompatible)
The Thermo Scientific Dionex Ultimate 3000 Bio-LC system is applied for the peptide mapping analysis of an Antibody Drug Conjugate (Kadcyla). The separation was performed on a Thermo Scientific Acclaim RSLC 120-C18 column by using a Thermo Scientific SMART digest kit at UV detection of 214 nm. With a run time of 55 minutes the method allows separation of all peptides of Kadcyla (Antibody Drug Conjugate). The SMART Digest kit provides significant improvements in reproducibility, which results in fewer sample failures, higher throughput and the ability to more easily interrogate data.
Selecting buffers to remove uncertainty in tryptic digestionInstrument Type: HPLC
The Thermo Scientific SMART Digest kit was used for the digestion of insulin. A variety of common buffer additives were compared to the SMART Digest buffer. Samples were analyzed on a Thermo Scientific Dionex UltiMate 3000 RSLC system via reversed-phase separation on a Thermo Scientific Accucore C18 column, with UV detection at 280 nm.
Improved analysis of phosphopeptides by automated IMAC in gallium form using a Thermo Scientific Acclaim 300 C18 HPLC columnInstrument Type: HPLC
The Thermo Scientific HPLC system is applied for the determination of phosphopeptides by automated IMAC in gallium. The separation is performed on a Thermo Scientific Acclaim 300 C18 HPLC column, with UV detection at 214 nm. Immobilized metal affinity chromatography (IMAC) is a powerful tool for enriching phosphopeptide isolates from protein digests. An automation package consisting of the Dionex dual-gradient LC system, ProPac IMAC-10 (for enrichment) and Acclaim 300 analytical column is the perfect solution for this technique.