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Improved analysis of phosphopeptides by automated IMAC in gallium form using a Thermo Scientific Acclaim 300 C18 HPLC column
Description
The Thermo Scientific HPLC system is applied for the determination of phosphopeptides by automated IMAC in gallium. The separation is performed on a Thermo Scientific Acclaim 300 C18 HPLC column, with UV detection at 214 nm. Immobilized metal affinity chromatography (IMAC) is a powerful tool for enriching phosphopeptide isolates from protein digests. An automation package consisting of the Dionex dual-gradient LC system, ProPac IMAC-10 (for enrichment) and Acclaim 300 analytical column is the perfect solution for this technique.| Market: | BioPharma |
| Keywords: | Acclaim 300 C18, Fractionation, HPLC, Phosphopeptides, ProPac IMAC-10, Trypsin digest, UV detection |
| Matrix: | N/A |
For Research Use Only. Not for use in diagnostic procedures.
