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Displaying 1-2 of 2 results for Tag: Phosphopeptide
Simple gradient analysis of phosphopeptides
Instrument Type: HPLCThe Thermo Scientific HPLC system is applied for the analysis of phosphopeptides. The sample of ß-casein was reduced and carboxymethylated, and digested for 9 hours with immobilized trypsin. The sample was fractionated by immobilized metal affinity chromatography (IMAC) in ferric form, before analysis using a Thermo Scientific Acclaim 300 C18 column, with UV detection at 214 nm. Note pyrophosphate is a UV transparent chelating agent that removes or masks interfering metal ions. The chromatograms shown are: A – fraction retained by IMAC B – unretained fraction C – unfractionated peptides
Improved analysis of phosphopeptides by automated IMAC in gallium form using a Thermo Scientific Acclaim 300 C18 HPLC column
Instrument Type: HPLCThe Thermo Scientific HPLC system is applied for the determination of phosphopeptides by automated IMAC in gallium. The separation is performed on a Thermo Scientific Acclaim 300 C18 HPLC column, with UV detection at 214 nm. Immobilized metal affinity chromatography (IMAC) is a powerful tool for enriching phosphopeptide isolates from protein digests. An automation package consisting of the Dionex dual-gradient LC system, ProPac IMAC-10 (for enrichment) and Acclaim 300 analytical column is the perfect solution for this technique.
