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Description
The Thermo Scientific HPLC system is applied for the analysis of phosphopeptides. The sample of ß-casein was reduced and carboxymethylated, and digested for 9 hours with immobilized trypsin. The sample was fractionated by immobilized metal affinity chromatography (IMAC) in ferric form, before analysis using a Thermo Scientific Acclaim 300 C18 column, with UV detection at 214 nm. Note pyrophosphate is a UV transparent chelating agent that removes or masks interfering metal ions. The chromatograms shown are: A – fraction retained by IMAC B – unretained fraction C – unfractionated peptides| Market: | BioPharma |
| Keywords: | HPLC, IMAC, Phosphopeptides, UV detection, beta-casein |
| Matrix: | N/A |
For Research Use Only. Not for use in diagnostic procedures.
