AN177: Separation of an Intact Monoclonal Antibody and Fractionation of Monoclonal Antibody Papain Digest Fragments Using IMAC: Papain Digest Separation

Instrument Type: IC

This application note describes a method for fractionating IgG1 MAb Fc and Fab fragments with the ProPac IMAC-10 column preloaded with copper and analyzing the fractions using the ProPac WCX-10 column. This record reports the method for the papain digest separation with the ProPac IMAC-10 column.

AN177: Separation of an Intact Monoclonal Antibody and Fractionation of Monoclonal Antibody Papain Digest Fragments Using IMAC: Separation of the Intact IgG

Instrument Type: IC

This application note describes a method for fractionating IgG1 MAb Fc and Fab fragments with the ProPac IMAC-10 column preloaded with copper and analyzing the fractions using the ProPac WCX-10 column. This record reports the separation method for the intact antibody.

AN177: Separation of an Intact Monoclonal Antibody and Fractionation of Monoclonal Antibody Papain Digest Fragments IMAC: Separation of IMAC Fractions

Instrument Type: IC

This application note describes a method for fractionating IgG1 MAb Fc and Fab fragments with the ProPac IMAC-10 column preloaded with copper and analyzing the fractions using the ProPac WCX-10 column. This record reports the method for the WCX-10 separation.

Improved analysis of phosphopeptides by automated IMAC in gallium form using a Thermo Scientific Acclaim 300 C18 HPLC column

Instrument Type: HPLC

The Thermo Scientific HPLC system is applied for the determination of phosphopeptides by automated IMAC in gallium. The separation is performed on a Thermo Scientific Acclaim 300 C18 HPLC column, with UV detection at 214 nm. Immobilized metal affinity chromatography (IMAC) is a powerful tool for enriching phosphopeptide isolates from protein digests. An automation package consisting of the Dionex dual-gradient LC system, ProPac IMAC-10 (for enrichment) and Acclaim 300 analytical column is the perfect solution for this technique.