Thermo Scientific AppsLab Library: AN177: Separation of an Intact Monoclonal Antibody and Fractionation of Monoclonal Antibody Papain Digest Fragments Using IMAC: Separation of the Intact IgG

Rate

Ask the Expert

Suggest an Application

Find methods for your needs

AN177: Separation of an Intact Monoclonal Antibody and Fractionation of Monoclonal Antibody Papain Digest Fragments Using IMAC: Separation of the Intact IgG Be the first to rate this application

$an177 separation an intact monoclonal antibody fractionation monoclonal antibody papain digest fragments using imac separation intact igg$

Description

This application note describes a method for fractionating IgG1 MAb Fc and Fab fragments with the ProPac IMAC-10 column preloaded with copper and analyzing the fractions using the ProPac WCX-10 column. This record reports the separation method for the intact antibody.

Market:	BioPharma
Keywords:	FAB, Fc, IgG, Immobilized Metal Affinity Chromatography, mAb, MAB, ProPac, ProPac IMAC-10, ProPac WCX-10
Matrix:	Protein
Author:	Brian De Borba and Jeff Rohrer
Affiliation:	Thermo Fisher Scientific

Uploaded on 8/19/2015.

For Research Use Only. Not for use in diagnostic procedures.

Find methods for your needs

AN177: Separation of an Intact Monoclonal Antibody and Fractionation of Monoclonal Antibody Papain Digest Fragments Using IMAC: Separation of the Intact IgG Be the first to rate this application

Description

Tags