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Displaying 1-5 of 6 results for Tag: ProPac WCX-10

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$an177 separation an intact monoclonal antibody fractionation monoclonal antibody papain digest fragments using imac papain digest separation$

AN177: Separation of an Intact Monoclonal Antibody and Fractionation of Monoclonal Antibody Papain Digest Fragments Using IMAC: Papain Digest Separation

Instrument Type: IC

This application note describes a method for fractionating IgG1 MAb Fc and Fab fragments with the ProPac IMAC-10 column preloaded with copper and analyzing the fractions using the ProPac WCX-10 column. This record reports the method for the papain digest separation with the ProPac IMAC-10 column.

$an177 separation an intact monoclonal antibody fractionation monoclonal antibody papain digest fragments using imac separation intact igg$

AN177: Separation of an Intact Monoclonal Antibody and Fractionation of Monoclonal Antibody Papain Digest Fragments Using IMAC: Separation of the Intact IgG

Instrument Type: IC

$an177 separation an intact monoclonal antibody fractionation monoclonal antibody papain digest fragments imac separation imac fractions$

AN177: Separation of an Intact Monoclonal Antibody and Fractionation of Monoclonal Antibody Papain Digest Fragments IMAC: Separation of IMAC Fractions

Instrument Type: IC

an130 identification a hydroxylysinecontainingpeptide using aaadirect

AN130: Identification of a Hydroxylysine-ContainingPeptide Using AAA-Direct

Instrument Type: HPLC (Biocompatible)

This application note shows that AAA-Direct can be used to determine peptides with lysine hydroxylation. All common amino acids, including hydroxylysine (Hyl), were separated by AAA-Direct, and the presence or absence of Hyl in the fractions confirmed peak identities. The techniques described in this application note show several ways that peptides can be evaluated for identity and purity, how peptides can be desalted, and how much desalting is needed for successful AAADirect amino acid analysis.

Monitoring protein deamidation using a Thermo Scientific ProPac WCX-10 column

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific UltiMate 3000 Titanium HPLC system is applied to monitor the products of protein deamidation. The separation is performed on a Thermo Scientific ProPac WCX-10 column, with UV detection at 280 nm.