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AN177: Separation of an Intact Monoclonal Antibody and Fractionation of Monoclonal Antibody Papain Digest Fragments IMAC: Separation of IMAC Fractions Be the first to rate this application

an177 separation an intact monoclonal antibody fractionation monoclonal antibody papain digest fragments imac separation imac fractions


This application note describes a method for fractionating IgG1 MAb Fc and Fab fragments with the ProPac IMAC-10 column preloaded with copper and analyzing the fractions using the ProPac WCX-10 column. This record reports the method for the WCX-10 separation.
Market: BioPharma
Keywords: FAB, Fc, IgG, Immobilized Metal Affinity Chromatography, mAb, MAB, ProPac, ProPac IMAC-10, ProPac WCX-10
Matrix: Protein
Author: Brian De Borba and Jeff Rohrer
Affiliation: Thermo Fisher Scientific
Uploaded on 8/18/2015.

For Research Use Only. Not for use in diagnostic procedures.